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溶壁酶(lywallzyme

<span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">溶壁酶使用说明书 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">  货号:BD-Lywallzyme 01 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">英文名称：Lywallzyme <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">其他名称：真菌细胞壁溶解酶 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">酶活性：原生质体产率≥1.0×106 个/mL<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">酶性状：灰白色或浅棕色粉末，易溶于水，活性稳定，较耐高温，便于贮存，产品质量稳定。 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">适用范围：酶解真菌细胞壁。对大多数真菌都有较好的脱壁效果，对担子菌和子囊菌的效果<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">*佳。其中食用和药用真菌，如草菇、平菇、蘑菇、香菇、银耳和灵芝等；工农业生产上的<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">重要菌种，如产黄青霉、黑曲霉、酵母等；研究遗传用的重要材料，如裂褶菌、粗糙脉孢菌、<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">构槽曲霉等，都能用溶壁酶去壁，获得大量原生质体，分离的原生质体活性强，有再生能力。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">用途：分解真菌细胞壁，可获得高产、优质的原生质体。该酶是真菌生物工程研究的有效工<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">具酶 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">保存方法：3 个月以内：4℃干燥保存；3 个月以上：-20℃干燥保存。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">包装规格：1 克/瓶 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">溶壁酶酶解真菌菌丝获得原生质体 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">参考方法 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">一、材料<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">1、供试菌株：凤尾菇（Pleurotus sajor-caju）。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">2、培养基：PD-马铃薯液体培养基。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">3、稳渗剂：0.6mol/LMgSO4 溶液或 0.8mol/L 甘露醇溶液。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">4、酶液：用稳渗剂配置含溶壁酶 15mg/mL 的酶液 3mL。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">二、方法<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">1、菌丝培养：供试菌株斜面种转入马铃薯液体培养基中，25℃恒温静置培养 6 天。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">2、菌丝处理：将培养好的菌丝用无菌纯水洗涤 2 次。再用稳渗剂洗涤 1 次，离心去水<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">分。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">3、酶解：取处理好的菌丝 300mg 至配好的酶液中，摇匀，置 30℃恒温水浴中水解反应<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">1.5h，反应过程间歇振摇（动作应温和）。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">4、原生质体收集：酶解结束后，酶解液用灭菌脱脂棉过滤。滤液于 2000rpm 离心 10min，<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">收集原生质体。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">5、原生质体计数：离心完成后弃上清液，沉淀即为原生质体。原生质体用稳渗剂定至 3mL，然后用血球计数法计算原生质体量。

￥1450.00

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color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">酶性状：灰白色或浅棕色粉末，易溶于水，活性稳定，较耐高温，便于贮存，产品质量稳定。 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">适用范围：酶解真菌细胞壁。对大多数真菌都有较好的脱壁效果，对担子菌和子囊菌的效果<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">*佳。其中食用和药用真菌，如草菇、平菇、蘑菇、香菇、银耳和灵芝等；工农业生产上的<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">重要菌种，如产黄青霉、黑曲霉、酵母等；研究遗传用的重要材料，如裂褶菌、粗糙脉孢菌、<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">构槽曲霉等，都能用溶壁酶去壁，获得大量原生质体，分离的原生质体活性强，有再生能力。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">用途：分解真菌细胞壁，可获得高产、优质的原生质体。该酶是真菌生物工程研究的有效工<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">具酶 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">保存方法：3 个月以内：4℃干燥保存；3 个月以上：-20℃干燥保存。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">包装规格：1 克/瓶 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">溶壁酶酶解真菌菌丝获得原生质体 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">参考方法 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">一、材料<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">1、供试菌株：凤尾菇（Pleurotus sajor-caju）。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">2、培养基：PD-马铃薯液体培养基。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">3、稳渗剂：0.6mol/LMgSO4 溶液或 0.8mol/L 甘露醇溶液。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">4、酶液：用稳渗剂配置含溶壁酶 15mg/mL 的酶液 3mL。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">二、方法<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">1、菌丝培养：供试菌株斜面种转入马铃薯液体培养基中，25℃恒温静置培养 6 天。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">2、菌丝处理：将培养好的菌丝用无菌纯水洗涤 2 次。再用稳渗剂洗涤 1 次，离心去水<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">分。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">3、酶解：取处理好的菌丝 300mg 至配好的酶液中，摇匀，置 30℃恒温水浴中水解反应<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">1.5h，反应过程间歇振摇（动作应温和）。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">4、原生质体收集：酶解结束后，酶解液用灭菌脱脂棉过滤。滤液于 2000rpm 离心 10min，<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">收集原生质体。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">5、原生质体计数：离心完成后弃上清液，沉淀即为原生质体。原生质体用稳渗剂定至 3mL，然后用血球计数法计算原生质体量。
[常用生化试剂]
头孢唑林钠Cefazol

2019-10-29

市场价: ￥45.00

价格: ￥45.00

头孢唑林钠 <span style="font: 400 14px/24px arial,sans-serif; text-align: left; color: rgb(51, 51, 51); text-transform: none; text-indent: 2em; letter-spacing: normal; text-decoration: none; word-spacing: 0px; display: inline !important; zoom: 1; orphans: 2; font-size-adjust: none; font-stretch: normal; float: none; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255);">  <span style="text-align: left; color: rgb(0, 0, 0); text-transform: none; line-height: 1.5; text-indent: 0px; letter-spacing: normal; font-size: 14px; display: inline !important; float: none; background-color: rgb(255, 255, 255);" sans="" inline="">英文名称: <span style="text-align: left; color: rgb(0, 0, 0); text-transform: none; line-height: 1.5; text-indent: 0px; letter-spacing: normal; font-size: 14px; display: inline !important; float: none; background-color: rgb(255, 255, 255);" sans="" inline="">Cefazolin sodium salt

3F 仪器及维修

原装进口

正品保证感恩回馈

PCR仪-博日
超声仪-宁波新芝

[常用国产耗材]
Y58S 灭菌乳胶手套5

2025-12-26

市场价: ￥1500.00

价格: ￥1300.00

 一次性医用灭菌橡胶检查手套医护外科检查加厚乳胶无菌独立包装【医用级】无粉麻面*1盒   Y58S 灭菌乳胶手套5.0g  2只/包，100包/盒10盒/箱   英科医疗产品  有：大，中，小
[耗材]
Y89 一次性加厚丁腈手

2025-12-26

市场价: ￥400.00

价格: ￥270.00

Y89 一次性加厚丁腈手套4.8g （100只/盒）(10盒一箱)    英科医疗产品  有：加大，大，中，小，小号
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SY98 一次性合成医用

2025-12-26

市场价: ￥400.00

价格: ￥280.00

 SY98 一次性合成医用乳胶检查手套4.5g （100只/盒）(10盒一箱)    英科医疗产品  有：加大，大，中，小，小号
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Y86 一次性医用丁腈手

2025-12-26

市场价: ￥300.00

价格: ￥230.00

 Y86 一次性医用丁腈手套4.0g（100只/盒）(10盒一箱)    英科医疗产品  有：加大，大，中，小，小号
[试剂]
溶壁酶(lywallzy

2022-12-20

市场价: ￥1450.00

价格: ￥1450.00

<span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">溶壁酶使用说明书 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">  货号:BD-Lywallzyme 01 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">英文名称：Lywallzyme <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">其他名称：真菌细胞壁溶解酶 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">酶活性：原生质体产率≥1.0×106 个/mL<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">酶性状：灰白色或浅棕色粉末，易溶于水，活性稳定，较耐高温，便于贮存，产品质量稳定。 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">适用范围：酶解真菌细胞壁。对大多数真菌都有较好的脱壁效果，对担子菌和子囊菌的效果<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">*佳。其中食用和药用真菌，如草菇、平菇、蘑菇、香菇、银耳和灵芝等；工农业生产上的<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">重要菌种，如产黄青霉、黑曲霉、酵母等；研究遗传用的重要材料，如裂褶菌、粗糙脉孢菌、<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">构槽曲霉等，都能用溶壁酶去壁，获得大量原生质体，分离的原生质体活性强，有再生能力。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">用途：分解真菌细胞壁，可获得高产、优质的原生质体。该酶是真菌生物工程研究的有效工<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">具酶 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">保存方法：3 个月以内：4℃干燥保存；3 个月以上：-20℃干燥保存。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">包装规格：1 克/瓶 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">溶壁酶酶解真菌菌丝获得原生质体 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">参考方法 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">一、材料<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">1、供试菌株：凤尾菇（Pleurotus sajor-caju）。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">2、培养基：PD-马铃薯液体培养基。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">3、稳渗剂：0.6mol/LMgSO4 溶液或 0.8mol/L 甘露醇溶液。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">4、酶液：用稳渗剂配置含溶壁酶 15mg/mL 的酶液 3mL。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">二、方法<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">1、菌丝培养：供试菌株斜面种转入马铃薯液体培养基中，25℃恒温静置培养 6 天。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">2、菌丝处理：将培养好的菌丝用无菌纯水洗涤 2 次。再用稳渗剂洗涤 1 次，离心去水<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">分。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">3、酶解：取处理好的菌丝 300mg 至配好的酶液中，摇匀，置 30℃恒温水浴中水解反应<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">1.5h，反应过程间歇振摇（动作应温和）。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">4、原生质体收集：酶解结束后，酶解液用灭菌脱脂棉过滤。滤液于 2000rpm 离心 10min，<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">收集原生质体。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">5、原生质体计数：离心完成后弃上清液，沉淀即为原生质体。原生质体用稳渗剂定至 3mL，然后用血球计数法计算原生质体量。
[常用生化试剂]
头孢唑林钠Cefazol

2019-10-29

市场价: ￥45.00

价格: ￥45.00

头孢唑林钠 <span style="font: 400 14px/24px arial,sans-serif; text-align: left; color: rgb(51, 51, 51); text-transform: none; text-indent: 2em; letter-spacing: normal; text-decoration: none; word-spacing: 0px; display: inline !important; zoom: 1; orphans: 2; font-size-adjust: none; font-stretch: normal; float: none; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255);">  <span style="text-align: left; color: rgb(0, 0, 0); text-transform: none; line-height: 1.5; text-indent: 0px; letter-spacing: normal; font-size: 14px; display: inline !important; float: none; background-color: rgb(255, 255, 255);" sans="" inline="">英文名称: <span style="text-align: left; color: rgb(0, 0, 0); text-transform: none; line-height: 1.5; text-indent: 0px; letter-spacing: normal; font-size: 14px; display: inline !important; float: none; background-color: rgb(255, 255, 255);" sans="" inline="">Cefazolin sodium salt

超声仪-宁波新芝

电泳仪-北京六一

PCR仪-博日

微生物学
生化试剂
有机溶液标准物质

[常用国产耗材]
Y58S 灭菌乳胶手套5

2025-12-26

市场价: ￥1500.00

价格: ￥1300.00

 一次性医用灭菌橡胶检查手套医护外科检查加厚乳胶无菌独立包装【医用级】无粉麻面*1盒   Y58S 灭菌乳胶手套5.0g  2只/包，100包/盒10盒/箱   英科医疗产品  有：大，中，小
[耗材]
Y89 一次性加厚丁腈手

2025-12-26

市场价: ￥400.00

价格: ￥270.00

Y89 一次性加厚丁腈手套4.8g （100只/盒）(10盒一箱)    英科医疗产品  有：加大，大，中，小，小号
[耗材]
SY98 一次性合成医用

2025-12-26

市场价: ￥400.00

价格: ￥280.00

 SY98 一次性合成医用乳胶检查手套4.5g （100只/盒）(10盒一箱)    英科医疗产品  有：加大，大，中，小，小号
[耗材]
Y86 一次性医用丁腈手

2025-12-26

市场价: ￥300.00

价格: ￥230.00

 Y86 一次性医用丁腈手套4.0g（100只/盒）(10盒一箱)    英科医疗产品  有：加大，大，中，小，小号
[试剂]
溶壁酶(lywallzy

2022-12-20

市场价: ￥1450.00

价格: ￥1450.00

<span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">溶壁酶使用说明书 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">  货号:BD-Lywallzyme 01 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">英文名称：Lywallzyme <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">其他名称：真菌细胞壁溶解酶 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">酶活性：原生质体产率≥1.0×106 个/mL<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">酶性状：灰白色或浅棕色粉末，易溶于水，活性稳定，较耐高温，便于贮存，产品质量稳定。 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">适用范围：酶解真菌细胞壁。对大多数真菌都有较好的脱壁效果，对担子菌和子囊菌的效果<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">*佳。其中食用和药用真菌，如草菇、平菇、蘑菇、香菇、银耳和灵芝等；工农业生产上的<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">重要菌种，如产黄青霉、黑曲霉、酵母等；研究遗传用的重要材料，如裂褶菌、粗糙脉孢菌、<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">构槽曲霉等，都能用溶壁酶去壁，获得大量原生质体，分离的原生质体活性强，有再生能力。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">用途：分解真菌细胞壁，可获得高产、优质的原生质体。该酶是真菌生物工程研究的有效工<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">具酶 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">保存方法：3 个月以内：4℃干燥保存；3 个月以上：-20℃干燥保存。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">包装规格：1 克/瓶 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">溶壁酶酶解真菌菌丝获得原生质体 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">参考方法 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">一、材料<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">1、供试菌株：凤尾菇（Pleurotus sajor-caju）。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">2、培养基：PD-马铃薯液体培养基。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">3、稳渗剂：0.6mol/LMgSO4 溶液或 0.8mol/L 甘露醇溶液。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">4、酶液：用稳渗剂配置含溶壁酶 15mg/mL 的酶液 3mL。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">二、方法<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">1、菌丝培养：供试菌株斜面种转入马铃薯液体培养基中，25℃恒温静置培养 6 天。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">2、菌丝处理：将培养好的菌丝用无菌纯水洗涤 2 次。再用稳渗剂洗涤 1 次，离心去水<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">分。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">3、酶解：取处理好的菌丝 300mg 至配好的酶液中，摇匀，置 30℃恒温水浴中水解反应<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">1.5h，反应过程间歇振摇（动作应温和）。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">4、原生质体收集：酶解结束后，酶解液用灭菌脱脂棉过滤。滤液于 2000rpm 离心 10min，<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">收集原生质体。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">5、原生质体计数：离心完成后弃上清液，沉淀即为原生质体。原生质体用稳渗剂定至 3mL，然后用血球计数法计算原生质体量。
[常用生化试剂]
头孢唑林钠Cefazol

2019-10-29

市场价: ￥45.00

价格: ￥45.00

头孢唑林钠 <span style="font: 400 14px/24px arial,sans-serif; text-align: left; color: rgb(51, 51, 51); text-transform: none; text-indent: 2em; letter-spacing: normal; text-decoration: none; word-spacing: 0px; display: inline !important; zoom: 1; orphans: 2; font-size-adjust: none; font-stretch: normal; float: none; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255);">  <span style="text-align: left; color: rgb(0, 0, 0); text-transform: none; line-height: 1.5; text-indent: 0px; letter-spacing: normal; font-size: 14px; display: inline !important; float: none; background-color: rgb(255, 255, 255);" sans="" inline="">英文名称: <span style="text-align: left; color: rgb(0, 0, 0); text-transform: none; line-height: 1.5; text-indent: 0px; letter-spacing: normal; font-size: 14px; display: inline !important; float: none; background-color: rgb(255, 255, 255);" sans="" inline="">Cefazolin sodium salt

[常用生化试剂]
头孢唑林钠Cefazol

2019-10-29

市场价: ￥45.00

价格: ￥45.00

头孢唑林钠 <span style="font: 400 14px/24px arial,sans-serif; text-align: left; color: rgb(51, 51, 51); text-transform: none; text-indent: 2em; letter-spacing: normal; text-decoration: none; word-spacing: 0px; display: inline !important; zoom: 1; orphans: 2; font-size-adjust: none; font-stretch: normal; float: none; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255);">  <span style="text-align: left; color: rgb(0, 0, 0); text-transform: none; line-height: 1.5; text-indent: 0px; letter-spacing: normal; font-size: 14px; display: inline !important; float: none; background-color: rgb(255, 255, 255);" sans="" inline="">英文名称: <span style="text-align: left; color: rgb(0, 0, 0); text-transform: none; line-height: 1.5; text-indent: 0px; letter-spacing: normal; font-size: 14px; display: inline !important; float: none; background-color: rgb(255, 255, 255);" sans="" inline="">Cefazolin sodium salt
[常用生化试剂]
MES 2-(N-吗啡啉

2019-10-29

市场价: ￥80.00

价格: ￥80.00

MES 2-(N-吗啡啉)乙磺酸 CAS号 145224-94-8  分子式 C6H13NO4S·H2O 分子量213.25
[生化试剂]
TRIzol Reage

2019-01-25

市场价: ￥2637.00

价格: ￥1978.00

Invitrogen<img style="background:url(script/ueditor/lang/zh-cn/images/localimage.png) no-repeat center center;border:1px solid #ddd" src="script/ueditor/themes/default/images/spacer.gif" word_img="file:///C:/Users/DELL/AppData/Local/Temp/%7BD41FVA2GN7OA2X26ICDLK9.png"/>  TRIzol<img style="background:url(script/ueditor/lang/zh-cn/images/localimage.png) no-repeat center center;border:1px solid #ddd" src="script/ueditor/themes/default/images/spacer.gif" word_img="file:///C:/Users/DELL/AppData/Local/Temp/%7BD41FVA2GN7OA2X26ICDLK9.png"/> Reagent
[常用生化试剂]
D-潘糖 D-Pano

2018-07-31

市场价: ￥1530.00

价格: ￥1530.00

 D-潘糖    潘糖     D-Panose      CAS：33401-87-5
[常用生化试剂]
Rifampicin利福

2018-07-27

市场价: ￥460.00

价格: ￥391.00

利福平 Rifampicin 别名: 3-(4-甲基-1-哌嗪基亚胺甲基)利福霉素SV, 利米定, 甲哌利福霉素 CAS号:13292-46-1
[常用生化试剂]
利福平Rifampici

2018-07-27

市场价: ￥120.00

价格: ￥120.00

利福平Rifampicin (Rifampin)    98%   Cas: 13292-46-1